Regulation of Mouse p45 NF-E2 Transcription by an Erythroid-specific GATA-dependent Intronic Alternative Promoter
نویسندگان
چکیده
منابع مشابه
Requirement of GATA-1 and p45 NF-E2 expression in butyric acid-induced erythroid differentiation.
Butyric acid (BA) is known to induce overexpression of fetal hemoglobin and then erythroid differentiation. Therefore, BA is currently under clinical investigation as a potential therapy for the treatment of sickle cell disease and cancer. Nevertheless, the molecular mechanisms involved in BA-induced differentiation remain largely unknown. Previous reports have shown that BA-induced overexpress...
متن کاملSelf-Regulation of Mouse p45/NF-E2 during Murine Erythroleukemia Cell Differentiation
Tung-Liang Lee, Shau-Ching Wen, Wei-Yuan Hsiao, Che-Kun James Shen, and Yu-Chiau Shyu (2009) Self-regulation of mouse p45/NF-E2 during murine erythroleukemia cell differentiation. Zoological Studies 48(3): 362-369. The complicated process of murine erythroleukemia (MEL) cell differentiation is precisely controlled by a group of transcription factors. One of those transcription factors, p45/NF-E...
متن کاملRegulation of the erythroid transcription factor NF-E2 by cyclic adenosine monophosphate-dependent protein kinase.
Activation of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (A-kinase) promotes hemoglobin synthesis in several erythropoietin-dependent cell lines, whereas A-kinase-deficient murine erythroleukemia (MEL) cells show impaired hemoglobin production; A-kinase may regulate the erythroid transcription factor NF-E2 by directly phosphorylating its p45 subunit or by changing p45 intera...
متن کاملThe distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal γ-globin genes
GATA-1 and NF-E2 are erythroid specific activators that bind to the β-globin locus. To explore the roles of these activators in transcription of the human fetal stage specific γ-globin genes, we reduced GATA-1 and p45/NF-E2 using shRNA in erythroid K562 cells. GATA-1 or p45/NF-E2 knockdown inhibited the transcription of the γ-globin genes, hypersensitive site (HS) formation in the LCR and chrom...
متن کاملMultiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells.
Regulated expression of genes in the beta-globin cluster depends upon sequences located between 5 and 20 kb upstream of the epsilon gene, known as the locus control region (LCR). beta-Globin expression in murine erythroleukemia (MEL) cells depends on NF-E2, a transcription factor which binds to enhancer sequences in the LCR. To gain insight into the mechanism of globin gene activation by NF-E2,...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.14.10567